Table 2.

Validated and Predicted Gene Targets of miRNAs of Interest

miRNA	Validated Targets	Predicted Targets
hsa-miR-20a-5p	BMP2, PDGFB, THBS1, VEGFA	ANGPT1, ICAM1, IGFBP3, NRG1, UBB
hsa-miR-23b-3p	—	ADIPOQ, ANGPT2, BMP2, CTGF, HGF1, NRG1, PDGFB, THBS1, VEGFA
hsa-miR-142-3p	—	ANGPT2, HGF, IGFBP3, NRG1, VEGFA
hsa-miR-185-5p	VEGFA	ADIPOQ, ANGPT2, BMP2, ICAM1, NOV, NRG1, PDGFB
hsa-miR-326	NOTCH1, NOTCH2	CTGF, GDF15, HGF, IGFBP2, IGFBP3, MMP2, MMP9, NGFR, NOTCH3, NRG1, PDGFA, PDGFB, PDGFRB, TIMP2, VEGFA
hsa-miR-362-5p	—	ADIPOQ, ANGPT1, ANGPT2, MMP2, NGFR, NRG1, PDGFB, PDGFRA, PDGFRB, THBS1, TIMP2

Validated and predicted targets of miRNAs of interest were identified using miRTarBase²⁹ and miRWalk,³⁰ respectively. The focus was on genes that are known to play a role in PDR and that are changed in the vitreous of PDR patients according to Klaassen et al.³¹