Figure 1.

Confirmation of the NIH-OVCAR3 cells as representative of HGSOC. (A) Mutation and copy number alteration data from the CCLE database was analysed. Mutations were categorised as homozygous (red) or heterozygous (blue) using a variant allele frequency cut-off of 0.8, mutations with variant allele frequency >0.8 were considered homozygous. The copy number alterations were categorised using their GISTIC scores of −2 or 2 (amplifications: GISTIC score of 2 (orange) and deletions: GISTIC score of −2 (purple). (B) RNA-seq read counts of 2426 genes from the CCLE database were correlated with the read counts for the same 2426 genes established from targeted RNA-sequencing to confirm authenticity of the NIH-OVCAR3 cells in our possession.