Skip to main content
. 2020 Jul 20;9(7):1735. doi: 10.3390/cells9071735

Figure 2.

Figure 2

Lamin A/C binds PCAF and HDAC2 in committed C2C12 cells. (a) Lamin A/C- HDAC2 PLA (red dots) in cycling and committed myoblasts or myotubes. Myogenin (green) was used as a marker of myogenic differentiation. (b) PCAF-lamin A/C PLA (red signal) in cycling myoblasts, committed myoblast and myotubes. (c) Lamin A/C-PCAF and lamin A/C -HDAC2 PLA (red dots) in representative myoblasts. Inset, high magnification of the area indicated by a rectangle. (d) PCAF-HDAC2 PLA (red dots) and immunofluorescence staining of myogenin (green) in cycling or committed myoblasts and in myotubes. Quantitative analysis of PLA signals is reported in the graphs. DAPI (blue staining) was used to counterstain cell nuclei. Scale bars, 10 µm. Three biological replicates were used in each experiment. Statistically significant differences between values (p < 0.05 or p < 0.01) are indicated.