Figure 5.
Western blotting of XH-14C to quantify ABCB1 expression level. (A) ABCB1 protein expression after incubation with different concentrations of XH-14C for 72 h in KB-C2 cells. KB-3-1 cells without any treatment used as the negative control of ABCB1 protein expression. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was used as loading control. (B) The relative intensity of the expression of ABCB1 compared with the expression of GAPDH. Expression level quantification by gray scale values calculated by ImageJ software (NIH, Bethesda, MD, USA). *, represents p < 0.05.
