Figure 2.

MAP infection delays maturation of moDCs. moDCs (1.8 × 10⁴ cells) were untreated or infected/treated with the same number of opsonized MAP, antibiotic-killed MAP (KMAP), MAP+LPS, or LPS alone for T = 0 (2 h), T = 24 h, and T = 48 h. MoDCs were stained for CD80, CD86, CD11b, CD11c, CD103, and MHCII and the mean fluorescence intensity (MFI) of CD80, CD86, MHCII, CD103, CD11b, and CD11c within the CD11c⁺CD11b⁺ population was calculated, and normalized to the MFI, from cells in the same experiment that were untreated. * significance vs. untreated: * p < 0.05; ** p < 0.01; *** p < 0.001; p < 0.0001 ****. # significance vs. MAP: # p < 0.05; ## p < 0.01. † Significance vs. KMAP: †† p < 0.01 (one-way ANOVA with multiple comparisons). Shown is the average ± SEM of n = 3–4 DC blood donors analyzed in triplicates.