Figure 3.

MAP-infected moDCs display a downregulated pro-inflammatory phenotype. MoDCs (1.8 × 10⁴ cells) were untreated or infected/treated with the same number of opsonized MAP, killed MAP (KMAP), or LPS alone for T = 0 (2 h), T = 24 h, and T = 48 h. Supernatants were collected and ELISA for IL-6, IL-10, and TNFα was performed. * p < 0.05; ** p < 0.01; *** p < 0.001; p < 0.0001 **** as calculated using two-way ANOVA with multiple comparisons; † p < 0.05, t-test. Shown is the average ± SEM of n = 3–4 DC blood donors analyzed in triplicate.