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. 2020 Jul 13;8(7):1039. doi: 10.3390/microorganisms8071039

Figure 1.

Figure 1

The interaction between polymorphonuclear neutrophils (PMNs) and Bacillus anthracis (hkBa) or anthrax peptidoglycan (PGN) is dependent on the complement opsonization of pathogen particles. (A) Flow cytometry gating strategy and exemplification of neutrophil purification (CD16high leukocytes). (B) Histogram overlay of FITC-labelled hkBa (left) and/or PGN (right) interaction with CD16high PMNs isolated from a median responsive individual, in the presence of compstatin, a C3 convertase inhibitor, or the immunodepletion of complement factors.