Figure 5.

Flow cytometry quantitation of myeloperoxidase (MPO) immunoreactivity in neutrophils stimulated with hkBa (A) or PGN (B) in the presence of compstatin, a C3 convertase inhibitor, or the immunodepletion of complement factors. Data are shown as mean ± SD of 10 independent donors and the depict intracellular MPO fluorescence (geometric mean) in CD16^high neutrophils (left) or the changes in MPO fluorescence after paired normalization to control MPO immunoreactivity in the absence of agonists (right panel). Statistically significant differences compared to bacteria and/or PGN-induced MPO immunoreactivity in the presence of the autologous serum (AHS, shaded) are depicted graphically (* p < 0.05; ** p < 0.01; *** p < 0.001), and were computed by repeated measures one-way ANOVA with Holm–Sidak’s multiple comparisons tests.