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Numbers and length of filopodia are decreased after fixation. (A) The same GFP-hyaluronan synthase 3 (HAS3) expressing cell before (live) and after fixation with 4% paraformaldehyde (fixed). (B) A higher magnification from one edge of the cell. FiloQuant measurement of (C) filopodia length and (D) relative density in live and fixed cells (n = 25 cells for both groups). *** p < 0.001. Unpaired t-test. Magnification bars = 10 µm.
