The pre-therapeutic mutational landscape of responders (n = 21) vs. non-responders (n = 31) to immunotherapy. Plot of clinical and genomic variants. Each column represents a separate patient. Each row represents, from top to bottom: mutational burden calculated as number of mutations per megabase, best response (RECIST criteria: from left to right complete and partial response; stable and progressive disease), gene variants enriched in responders (NFKBIE, SCN1A, ANKRD30A, and NRG3), variants in NFKBIE-related genes as identified by STRING v10, see methods (PPP6R2, PPP6C, NFKB1, PPP6R1, RELA, and NFKB2), common melanoma genes (BRAF, NRAS, and NF1). Patient clinical characteristics are described, from top to bottom as: type of tissue assayed (primary or metastatic lesion), primary type of melanoma (superficial spreading, nodular, acral, mucosal, desmoplastic, unknown primary, not otherwise specified, other, and unknown). Note in particular the genes enriched in responders including NFKBIE and NFKBIE-related genes. The common melanoma genes are evenly distributed between the groups.