Table 1.
Method | Number of patients | Threshold | Reference |
---|---|---|---|
IHC | |||
80 UC | 0.01 cells/mm2 | [82] | |
114 UC | 1 inclusion body in histology, positive specific IHC staining for CMV in 10–20 fields of colonic biopsies |
[55] | |
13 CD, 18 UC | semiquantitative ranging from 1 to 3 (1=rare, 2=common and easy to find, and 3=numerous cells positive for CMV) |
[83] | |
30 UC, 8 CD | semiquantitative ranging from 1 to 3 (1 = rare, 2 = common and easy to find, and 3 = numerous cells positive for CMV) |
[84] | |
95 UC | ≥ 5 IHC positive cells/biopsy | [85] | |
46 UC | colectomy was only associated with > 2 positive cells/biopsy | [86] | |
257 UC | semiquantitative low grade (1 to 4 inclusion bodies per section) high grade (at least 5 inclusion bodies per section) |
[70] | |
qPCR | |||
30 UC | 10 copies/µg of DNA | [87] | |
42 UC | 10 copies/mg of tissue | [44] | |
40 IBD | > 1000 copies/100,000 cells | [80] | |
149 UC | 5 IU/100,000 cells | [81] | |
92 UC, 9 CD | ≥ 316 copies/mg of DNA | [64] | |
75 IBD | 10,000 copies/µg of DNA | [88] | |
103 UC, 30 CD | > 600 copies/100 000 cells of tissue | [45] | |
47 IBD | > 250 copies/mg of tissue | [42] |
IHC: Immunohistochemistry, qPCR: Quantitative polymerase chain reaction, UC: Ulcerative colitis, CD: Crohn disease, IBD: Inflammatory bowel disease, IU: International unit.