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Recombinant B7-H6 is stabilized by the C212S mutation. (a) Size-exclusion chromatography (SEC) profiles of recombinantly expressed wild-type B7-H6 and its C212S mutant. (b) Fractions of the wild-type B7-H6 SEC peak were analyzed by 15% SDS-PAGE under non-reducing (left) and reducing (right) conditions. (c) Sedimentation analysis of wild-type B7-H6 at 0.5 mg/mL shown as continuous size distribution of the sedimenting species c(s). Dimeric species are marked with an asterisk in (b,c).
