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SDS-PAGE analysis of deglycosylated NKp30_LBD and B7-H6 C212S mutant. Different glycosylation states are marked as WT for proteins expressed in HEK293T cells with wild-type (WT) N-glycosylation; GnTI− expressed in HEK293S GnTI− cells lacking N-acetylglucosaminyltransferase I activity which have, therefore, uniform Asn-GlcNAc2Man5 N-glycans; and DEG for the GnTI− proteins deglycosylated (DEG) with endoglycosidase Endo F1 (itself marked by an arrow).
