Figure 5.

Glycosylation is necessary for NKp30 oligomerization. (a) SEC-MALS analysis of NKp30_Stalk and NKp30_LBD, confirming that the stalk region is required for oligomerization. (b) SEC-MALS analysis of recombinantly expressed NKp30_Stalk with wild-type glycosylation (black), uniform, simple glycans (blue), both showing non-covalent oligomers, and deglycosylated sample, which does not form oligomers (red). (c) Normalized continuous size distributions of sedimenting species for glycosylated and deglycosylated NKp30_Stalk oligomers and for its monomeric fraction. The main peak corresponds to the NKp30_Stalk monomer, whereas a broad distribution of oligomeric species is present in glycosylated NKp30_Stalk samples.