Figure 5.

Intrastromal injection with HAse transiently decreased the interfibrillar spacing between the stromal collagens. (A) Increased packing of collagen fibrils was observed by TEM at days 1 and 7 in HAse-injected corneas versus BSS-treated and untreated controls; interfibrillar spacing returned to normal at day 90 in the HAse-treated group. (B) Quantitative analysis of the collagen spacing confirmed the aforementioned findings. (C) No change was observed in the diameter of the collagen fibrils throughout the study in all 3 groups. Results are expressed as mean ± SD. ⁺P < 0.05 compared to baseline, *P < 0.05 in between days 1 and 7 in the same group, ^#P < 0.05 between the HAse- and BSS-treated groups at the same time point, 1-way ANOVA, followed by Tukey's pairwise multiple comparison test.