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. 2020 Jul 30;11:1683. doi: 10.3389/fmicb.2020.01683

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Copyright © 2020 Lei, Chen, Kiba, Hikichi, Zhang and Ohnishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virulence assay of R. solanacearum T3Es mutants with combined deletion of four families in tobacco plants with leaf infiltration. Virulence of OE1-1 was compared with that of (A) RK7075 (Dgala Dskwp, OE1-1D13E), (B) RK7083 (Dgala Dskwp Dhlk, OE1-1D16E), and (C) RK7113 (Dgala Dskwp Dhlk Dawr, OE1-1D21E). Wilt symptoms were inspected daily and scored on a disease index scale from 0 to 4. Each assay was repeated with at least four biological replicates using 12 plants per trial. The mean values of all experiments were averaged with SE. A t-test was conducted each day, and asterisks indicated a significant difference (p < 0.05).