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. 2020 Jul 1;12(7):1756. doi: 10.3390/cancers12071756

Figure 2.

Figure 2

ptx3b downregulation causes left-right asymmetry defects in zebrafish embryo. (A) RT-PCR analysis showing the effect of different doses of ctrl or ptx3b morpholino (MO) on ptx3b expression in 28 hpf injected embryos. The efficacy of ptx3b MO is demonstrated by the presence of a specific 212 bp band in ptx3b MO-injected embryos, which confirms the occurrence of exon skipping. ef1a serves as control; (B) percentage of embryos showing normal, moderate, or severe phenotype at 72 hpf after the injection of 0.6 pmoles of ctrl MO or ptx3b MO, respectively; (C) representative bright field whole mount pictures of the phenotypes observed in ctrl and ptx3b morphants; (D) WISH representative pictures of the alterations of the expression of the laterality genes spaw and pitx2 observed in ptx3b morphants at 16 ss and 22 hpf, respectively; (E) percentage of embryos with normal or altered expression of spaw and pitx2 in WT (untreated) and ptx3b MO-injected embryos. Data are the mean of 4 and 3 independent experiment, respectively; (F) WISH representative pictures of cmlc2 expression during normal and altered cardiac jogging and looping at 26 and 48 hpf, respectively; (G) percentage of embryos with normal or altered cardiac jogging and looping in ctrl MO and ptx3b MO-injected embryos. Data are the mean of 2 and 3 independent experiments, respectively. * p < 0.05, *** p < 0.001, ANOVA. n, total number of analyzed embryos.