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. 2020 Jul 1;12(7):1756. doi: 10.3390/cancers12071756

Figure 4.

Figure 4

ptx3b regulates primary cilium length in zebrafish via the fibroblast growth factor (FGF)/FGF receptor (FGFR) system. (A) Western blot analysis of protein extracts from WT (untreated), ctrl MO, ptx3b MO, and ptx3b MO plus hPTX3 mRNA-injected embryos probed with an anti-pFGFR1 antibody; uniform loading of the gel was confirmed using an anti-α tubulin antibody; (B) densitometric analysis of pFGFR1 levels normalized to α-tubulin expression. Data are the mean ± S.E.M. of 3 independent experiments, ** p < 0.01, * p < 0.05, Student’s t-test; (C–E) representative images of the acetylated α-tubulin+ KV ciliary axoneme of 10 ss embryos injected with ctrl MO or ptx3b MO and of ptx3b MO-injected embryos treated at shield stage with BGJ398 (50 nM). Scale bar: 10 µm; (F) primary cilium length (in µm) in the KVs of embryos treated as in (C–E). Each dot is the mean length of primary cilia measured in a single KV. n, total number of examined KVs. Data are from three independent experiments, *** p < 0.001, Student’s t-test.