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. 2020 Jul 1;12(7):1756. doi: 10.3390/cancers12071756

Figure 6.

Figure 6

Inhibition of FGF signaling increases the length of primary cilium in TRAMP-C2 cells. (A–E) Cilia were visualized in serum-starved cells by acetylated α-tubulin immunostaining and their length was measured using the ImageJ software. Black dots represent individual cilia; red bars show the mean values. Data were obtained from three independent experiments, *** p < 0.001, Student’s t-test; (A) primary cilium length in TRAMP-C2 cells overexpressing the C-terminal or the N-terminal fragment of human PTX3; (B) primary cilium length in mock_TRAMP-C2 and N-term-hPTX3-TRAMP-C2 cells treated for 48 h with 30 ng/mL FGF2; (C) primary cilium length in TRAMP-C2 cells treated for 48 h with recombinant PTX3 protein (66 nM) or with anti-FGFR1 single-chain antibody fragment scFv-RR-C2 (300 nM); (D) primary cilium length in TRAMP-C2 cells treated for 48 h with the tyrosine kinase FGFR inhibitors PD173074 (100 nM), SU5402 (100 nM), or BGJ398 (100 nM); (E) primary cilium length in TRAMP-C2 treated for 48 h with the MAPK inhibitors PD98059 (10 µM) or U0126 (1.0 µM) or with the PI3K inhibitors LY294002 (10 µM) or perifosine (1.0 µM).