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. 2020 Jul 1;12(7):1756. doi: 10.3390/cancers12071756

Figure 7.

Figure 7

Inhibition of the FGF/FGFR system affects cilium-related signaling. (A) Serum-starved mock and hPTX3-TRAMP-C2 cell protein extracts were probed with an anti-trichoplein (TCHP) antibody. Uniform loading of the gel was assessed by probing the membrane with an anti-β actin antibody; (B) densitometric analysis of trichoplein levels normalized to β actin; (C) serum-starved mock-TRAMP-C2 cells were treated with the FGFR inhibitors BGJ398 (100 nM) or SU5402 (100 nM) for 48 h. After lysis, the extracts were probed with an anti-trichoplein antibody; (D) densitometric analysis of trichoplein levels normalized to β-actin; (E) Gli1 expression in serum-starved mock and hPTX3_TRAMP-C2 cells; (F) serum-starved hPTX3-TRAMP-C2 cells were incubated with recombinant FGF2 (30 ng/mL) for 1, 6, or 12 h. Then, Gli1 expression was evaluated by qRT-PCR and normalized to Gaphd mRNA levels. All data are the mean ± S.E.M. of 3–4 independent experiment, * p < 0.05, ** p < 0.01, Student’s t-test.