Table 1.

Primer and probe sequences used in real-time PCR assays for the detection of Brucella spp., B. abortus, B. melitensis and B. suis in camel sera, Egypt.

Target	Primer and Probe Sequences		Reference
Brucella spp.	5′-GCT CGG TTG CCA ATA TCA ATG C-3´	Forward	[25]
	5′-GGG TAA AGC GTC GCC AGA AG-3´	Reverse
	6-FAM-AAA TCT TCC ACC TTG CCC TTG CCA TCA-MGB	Probe
B. abortus	5′-GCG GCT TTT CTA CGG TAT TC-3´	Forward
	5′-CAT GCG CTA TGA TCT GGT TAC G-3´	Reverse
	Hex-CGC TCA TGC TCG CCA GAC TTC AAT G-BHQ1	Probe
B. melitensis	5′-AAC AAG CGG CAC CCC TAA AA-3´	Forward
	5′-CAT GCG CTA TGA TCT GGT TAC G-3´	Reverse
	Cy5-CAG GAG TGT TTC GGC TCA GAA TAA TCC ACA-BHQ2	Probe
B. suis	5′-GCC AAA TAT CCA TGC GGG AAG-3´	Forward	[26]
	5′-TGG GCA TTC TCT ACG GTG TG-3´	Reverse
	VIC-TTG CGC TTT TGT GAT CTT TGC TTA TGG-MGB	Probe