Table 2.
Seroprevalence and molecular identification of Brucella DNA in camel sera collected from Giza, Aswan and Al-Bahr Al-Ahmar (the Red Sea) governorates, Egypt.
| Governorate | Sex | Number of Samples | Seroprevalence n (%) | Molecular Identification | |||||
|---|---|---|---|---|---|---|---|---|---|
| RBPT * | i-ELISA * | c-ELISA * | CFT * | Real-Time-PCR n (%) |
Brucella spp. DNA Identification | Cq/Ct-Values ** | |||
| Giza | male | 55 | 11 (20.0) | 17 (30.9) | 7 (12.7) | 28 (50.9) | 3 (5.5) | 3 B. abortus | 37, 36, 35 |
| female | 51 | 4 (7.8) | 7 (13.7) | 8 (15.7) | 5 (9.8) | 6 (11.8) | 6 B. abortus | 30, 32, 33, 36, 37 | |
| Sub-total | 106 | 15 (14.2) | 24 (22.6) | 15 (14.2) | 33 (31.1) | 9 (8.5) | |||
| Aswan | male | 161 | 30 (18.6) | 41 (25.5) | 39 (24.2) | 51 (31.7) | 7 (4.4) | 6 B. abortus | 37, 37, 36, 36, 36, 36 |
| 1 B. melitensis | 34 | ||||||||
| female | 25 | 3 (12.0) | 7 (28.0) | 2 (8.0) | 8 (32.0) | 1 (4.0) | 1 B. abortus | 36 | |
| Sub-total | 186 | 33 (17.7) | 48 (25.8) | 41 (22.0) | 59 (31.7) | 8 (4.3) | |||
| Al-Bahr Al-Ahmar (the Red Sea) | male | 79 | 11(13.9) | 13 (16.5) | 20 (25.3) | 24 (30.4) | 14 (17.7) | 8 B. abortus | 37, 35, 31, 37, 35, 32, 36, 35 |
| 1 B. melitensis | 36 | ||||||||
| 5 B. suis | 37, 36, 33, 28, 37 | ||||||||
| female | 10 | 0 (0.0) | 2 (20.0) | 1 (10.0) | 2 (20.0) | 1 (10.0) | 1 B. abortus | 37 | |
| Sub-total | 89 | 11 (12.4) | 15 (16.9) | 21 (23.6) | 26 (29.2) | 15 (16.9) | |||
| Grand-total | 381 | 59 (15.5) | 87 (22.8) | 77 (20.2) | 118 (31.0) | 32 (8.4) | |||
* RBPT: Rose-Bengal plate test; i-ELISA: indirect ELISA; c-ELISA: competitive ELISA; CFT: complement fixation test. ** Cq/Ct-values: cycle quantification/ cycle threshold values.