Table 4.
Optimization results of iMK1321 model for 2,3-butanediol production with the MiMBL algorithm.
| Substrate | Production of 2,3-Butanediol (mmol gDCW−1 h−1) | Growth Rate (h−1) | Genetic Modifications ** | Reaction Modifications * |
|---|---|---|---|---|
| Glucose | 5.69 | 0.32 | EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTb = 0.03125 ACONTa = 0.03125 |
| EP10_02485 = 0.03125 | FEROpp = 0.03125 R00092 = 0.03125 |
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| Glucose | 5.17 | 0.293 | EP10_09595 = 0.03125 | R0467 = 0.515625 R00014 = 0.7578125 FEROpp = 0.03125 R03050 = 0.515625 |
| EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Arabinose | 6.32 | 0.35 | KFX31147.1 = 16.0 | URIDK3 = 8.5 URA6_1 = 4.75 |
| KFX31511,1 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Arabinose | 6.30 | 0.35 | EP10_02065 = 0.03125 | GDH2 = 0.03125 GLUDy = 0.03125 |
| KFX31511.1 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Xylose | 5.95 | 0.34 | EP10_12945 = 4.0 | PGM = 2.5 |
| EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Xylose | 5.95 | 0.34 | EP10_12945 = 4.0 | PGM = 2.5 |
| KFX31511.1 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Glycerine | 6.55 | 0.357 | EP10_13815 = 0.03125 | FBA = 0.03125 FBA2 = 0.03125 FBA3 = 0.03125 |
| EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Glycerine | 6.55 | 0.357 | EP10_13815 = 0.0625 | FBA = 0.0625 FBA2 = 0.0625 FBA3 = 0.0625 |
| EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
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| Glycerine | 6.46 | 0.359 | EP10_10240 = 0.03125 | R01056 = 0.03125 |
| EP10_15190 = 0.03125 | ACO1 = 0.03125 ACONTa = 0.03125 ACONTb = 0.03125 |
* Value less than 1—a decrease in the flux value through the reaction compared to wildtype. ** Value less than 1 is a gene knockout. while a value greater than 1 is a gene overexpression. A value greater than 1 is an increase in the flux value through the reaction compared to the wildtype. FEROpp—ferrooxidase; ACO1—Hydroxymutase Citrate; ACONTa—aconitase (citrate hydro-lyase); ACONTb—aconitase (hydrolysis isocitrate); R00092—Fe2 + NAD + oxidoreductase; R0467—(S)-2- Aceto-2-hydroxybutanoate pyruvate lyase; R03050—2-Acetolactate pyruvate lyase; R00014—Thiamine diphosphate, acetaldehyde transferase; URIDK3—dUMP phosphotransferase; URA6_1—UMP phosphotransferase; GDH2—l-Glutamate NAD + oxidoreductase; GLUDy—l-Glutamate dehydrogenase; PGM—phosphoglyceratmutase; FBA—fructose bisphosphate aldolase; FBA2-D—Fructose 1-phosphate D-glyceraldehyde 3-phosphate lyase; FBA3—Sedoheptulose-1,7-bisphosphate D—glyceraldehyde-3-phosphate lyase; R01056-D—ribose-5-phosphate-aldose-ketose-isomerase.