Figure 2.
Effect of γ-secretase inhibitor (DAPT) on Notch-1 signaling. (A–E): Notch-1, IL-6, MCL-1 and HES-1 expression was measured in macrophages that were pre-treated with DAPT (0–40 μM). (F–J): Expression levels of Notch-1, IL-6, MCL-1 and HES-1 in THP-1-derived macrophages that were pre-treated with DAPT then infected with MAP (UCF 4, 107 CFU/mL) for 24 h. The gene expression levels were measured by RT-PCR. IL-6 protein level was measured using ELISA. All experiments were performed in triplets. Data are shown as mean ± SD, and significance is considered as * p < 0.05.