(A) RT-qPCR analysis showing the levels of MchI, MhcIIa, MhcIIb, and MhcIIx transcripts in tibialis anterior muscle of 30-d-old control or Sam68−/− mice normalized for the levels of the housekeeping gene CypA (n = 3; means ± SEM); P-value was determined by t test (*P < 0.05). (B) Immunostaining for slow myosin heavy chain (slMHC; green), laminin (red), and Hoechst (blue) of cross section from 30-d-old mice; scale bar: 50 μM; magnification: 10× and 20×. (C) Quantification of slMHC-positive muscle fibers in tibialis anterior muscle from 30-d-old mice (n = 3; means ± SEM); P-value was determined by t test (*P < 0.05).