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. 2020 Jul 27;26:e922032-1–e922032-10. doi: 10.12659/MSM.922032

Figure 3.

Figure 3

Overexpression of miR-133a promotes the proliferation of cell models of MPP+-induced PD. (A) Cell viability was detected at 0, 24, 48, and 72 hours using the MTT assay. (B) The number of colonies in each group was detected using the colony formation assay. (C) EdU-positive cells in each group were measured using the EdU labeling assay. * Compared with the blank group, * P<0.05 and ** P<0.01; # P<0.05. Replicates=3. Data shown in panel A were analyzed using 2-way ANOVA, and data shown in panels B and C were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. MPP+ – 1-methyl-4-phenylpyridinium ion; MTT – 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PD – Parkinson’s disease; EdU – 5-ethynyl-2′-deoxyuridine.