Figure 5.

Overexpression of miR-133a inhibits the autophagy of cell models of MPP⁺-induced PD. (A) Western blots showing the levels of autophagy-related proteins. (B) Representative images of LC3 spots detected using immunofluorescence. (C) Representative images of autophagosomes in cells after different treatments captured using a transmission electron microscope. ** P<0.01. Replicates=3. Data presented in panel A were analyzed using 2-way ANOVA, and data presented in panel B were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. MPP⁺ – 1-methyl-4-phenylpyridinium ion; PD – Parkinson’s disease.