Figure 6.

MiR-133a targets RAC1. (A) Bioinformatics software and a website were used to predict the binding site for miR-133a in the 3′UTR of RAC1. (B) Fluorescence intensity of RAC1, as detected using the dual luciferase reporter gene assay. (C, D) Relative levels of the RAC1 mRNA and protein were detected using RT-qPCR and western blot analysis. ** P<0.01. Replicates=3. Data shown in panel B were analyzed using 2-way ANOVA, and data shown in panels C and D were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. miR – microRNA, RAC1 – Ras-related C3 botulinum toxin substrate 1; mRNA – messenger RNA; RT-qPCR – reverse transcription quantitative polymerase chain reaction.