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. 2020 Aug 5;12:66. doi: 10.1186/s13098-020-00573-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression of differentiation markers in hASC, IPC and GPC by indirect immunofluorescence. The images shown are representative of n = 3 experiments, visualized by confocal microscopy. Nuclear staining was performed with Hoechst 1: 500 (blue). Markers analyzed were Pdx1 (red), Ngn3 (green), Insulin (green), Glucagon (red), CD44 (green) and Vimentin (green). a Markers analyzed in hASC. b Markers analyzed in IPC. c Markers analyzed in GPC. Scale bar = 20 μm. Pdx1 pancreatic and duodenal homeobox 1, Ngn3 neurogenin 3, Ins insulin, Gcg glucagon, Vim vimentin