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. 2020 Jul 30;8:573. doi: 10.3389/fcell.2020.00573

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Copyright © 2020 Chivero, Liao, Niu, Tripathi, Tian, Buch and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of dendritic cell-derived EVs: Expression levels of dicer (A) and Lamp2b (B) in mouse DC2.4 dendritic cell line co-transfected with dicer siRNA and RVG-Lamp2b plasmid. (C) Extracellular vesicles (EVs) were isolated from the culture supernatants of engineered DC2.4 mouse dendritic cells by a series of centrifugation. (D) Zeta view count of EVs. (E) Size distribution of EVs. (F) Western blot images for the expression of CD63 and Lamp2 in purified EVs. All experiments were done at least three independent times, and representative figures are shown. Actin served as a loading control for western blots in B. ^∗p < 0.05 vs. control.