FIGURE 4.

Intranasal delivery of dendritic cell-derived EVs loaded with Cy5-miR-124 inhibits cocaine-mediated upregulation of TLR4, MYD88, STAT3 and NF-kB p65 in vivo. Wild-type C57BL/6N, mice were administered either cocaine (20 mg/kg; i.p.) or saline for seven consecutive days. On the fifth, sixth, and seventh days, mice were intranasally administered dendritic cell-derived EVs loaded with Cy5-miR-124 or Cy5-scrambled miRNAs or were left unloaded, followed by cocaine injection. (A–D) Western blot images depicting the effect of EV-miR-124 on the expression of TLR4, MYD88, STAT3 and NF-kB p65. Actin served as a loading control. Quantification of western blots is shown under each blot. Data are shown as mean ± SEM (n = 4/group, ^∗p < 0.05 vs. saline+ EV control, ^#p < 0.05 vs. cocaine+ EV control group).