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. 2020 Jul 30;8:542. doi: 10.3389/fcell.2020.00542

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Copyright © 2020 Marchesini, De Francesco, Mattioli-Belmonte, Zingaretti, Riccio, Orlando, Zavan and Riccio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Figure showed a sample of surgical procedure. (A) Monoliteral cutaneous incision in the dorsal paravertebral region; (B) Pocket preparation between subcutaneous connective layer and the Dorsalis Magnus muscle fascia; (C) Injection at different concentrations of talc into the pocket; (D) Skin closure of pocket to avoid talc overflow; (E) at 2 weeks, palpatory, in the injection site there is no subcutaneous granuloma formation (the rats were not sacrificed); (F–H) at 4 weeks, palpatory, in the injection site, there is subcutaneous granuloma formation and after sacrificing the rat, evidence of subcutaneous granuloma formation is observed.