KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-SORLA | Abcam | Cat#ab190684 |
| Purified mouse monoclonal anti-LR11 (clone 48) | BD Biosciences | Cat#611860; RRID: AB_399340 |
| Mouse monoclonal anti-Actin (clone C4) | EMD Millipore Corp | Cat#MAB1501; RRID: AB_2223041 |
| Mouse monoclonal anti-EEA1 | BD Biosciences | Cat#610456; RRID: AB_397829 |
| Mouse monoclonal anti-APP (clone 22C11) | Thermo Fisher Scientific | Cat#14-9749-80; RRID: AB_2572977 |
| Rabbit monoclonal anti-APP (clone Y188) | Abcam | Cat#ab32136; RRID: AB_2289606 |
| Mouse monoclonal anti-Rab5 | Synaptic Systems | Cat#108011; RRID: AB_887773 |
| Chicken polyclonal anti-Map2 | Abcam | Cat#ab92434; RRID: AB_2138147 |
| Mouse monoclonal anti-Nestin (clone 10c2) | Santa Cruz Biotechnology | Cat#sc23927; RRID: AB_627994 |
| Mouse monoclonal anti-TGN38 (clone B-6) | Santa Cruz Biotechnology | Cat#sc166594; RRID: AB_2287347 |
| Mouse monoclonal anti-Rab7 (clone Rab7-117) | Abcam | Cat#ab50533; RRID: AB_882241 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LY2886721 BACE1 Inhibitor | MedChemExpress | Cat#HY13240 |
| Critical Commercial Assays | ||
| Abeta V-PLEX Panel ELISA | Meso Scale Discovery | Cat#K15200E-2 |
| sAPPα/sAPPβ ELISA | Meso Scale Discovery | Cat#K15120E-1 |
| Experimental Models: Cell Lines | ||
| Human: Wildtype clone A6 iPS cell line | This paper | N/A |
| Human: Wildtype clone A7 iPS cell line | This paper | N/A |
| Human: SORL1 Knockout clone E1 iPS cell line | This paper | N/A |
| Human: SORL1 Knockout clone E4 iPS cell line | This paper | N/A |
| Oligonucleotides | ||
| gRNA targeting sequence: ATTGAACGACATGAACCCTC | This paper | N/A |
| ssODN: GGGAATTGATCCCTATGACAAACCAAATA CCATCTACATTGAACGACATGAACCCTCTGGCTA CTCCACGTCTTCCGAAGTACAGATTTCTTCCAG TCCCGGGAAAACCAGGAAG | This paper | N/A |
| SORLA: Forward primer ctctatcctgagtcaaggagtaac | This paper | N/A |
| SORLA: Reverse primer ccttccaattcctgtgtatgc | This paper | N/A |
| Software and Algorithms | ||
| CellProfiler | McQuin et al., 2018 | https://cellprofiler.org/ |
| ImageJ/FIJI | Schindelin et al., 2012 | https://imagej.nih.gov/ij/ |
| JACoP plugin | Bolte and Cordelières., 2006 | https://imagejdocu.tudor.lu/_media/plugin/analysis/jacop_2.0/just_another_colocalization_plugin/jacop_.jar |