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. 2020 Jul 31;3(10):e202000817. doi: 10.26508/lsa.202000817

Figure S3. Additional validation of molecular target.

Figure S3.

(A) Comparative sandwich immunoassays for histidine-rich glycoprotein (HRG). To confirm the molecular binding of HPA045005 to HRG, dilution of spiked-in HRG protein levels were studied. Different colors represent additional capture antibodies, such as the anti-HRG binder HPA054598. All were used for a multiplexed assessment of HRG binding. For the detection, a labeled version of HPA054598 was used. (B) High-density protein array analysis of HPA045005. The signal intensities from the interaction of the antibody with any of the 16,728 immobilized unique protein fragments on the glass slide are illustrated. For the antibody HPA045005, one peak was exclusively observed for the protein fragment that had been used for the production of the antibody (red bar), as well as for the positive controls detecting the presence of the rabbit IgG antibody (orange and green bars).