Fig. 1.

Phylogenetic tree and alignment of GSTs. a A phylogenetic tree comprising GSTs was constructed based on amino acid sequences according to the neighbor-joining method (1000 bootstrap replicates) with the CLUSTALW (Thompson et al. 1994) and MEGA programs. Blue asterisks indicate anthocyanin-related GSTs. b Alignment of anthocyanin-related GSTs with CLUSTALW and Jalview (version 2.10.3). Asterisks indicate the positions of conserved amino acid residues specific to anthocyanin-related GSTs. The red box indicates the three deleted amino acid residues due to the deletion in allele 1 of GST1 genome-edited line #3. The accession numbers of GSTs used for constructing the phylogenetic tree and the sequence alignment are listed in Additional file 1: Table S4