Fig. 2.

Schematic diagram of GtGST1. a Schematic diagram of the GST1 genomic structure. White boxes indicate the exons. Arrows indicate the primer positions. Details regarding the primers are listed in Additional file 1: Table S3. Shaded red boxes represent target sites 1 and 2 for the genome editing with the CRISPR/Cas9 system. b Third exon sequence containing target sites 1 and 2 for genome editing. Shaded single nucleotides are the SNPs between two alleles. Bold nucleotides represent the sequences of target sites 1 and 2, and the adjacent blue shaded boxes (PAM) indicate the protospacer adjacent motif (NGG). Arrows indicate the primer positions