Fig. 4.

Analysis of anthocyanin accumulation in the petals of GST1 genome-edited gentian lines. a Chromatogram at 530 nm for the anthocyanins in petal extracts from WT plants and GST1 genome-edited lines. b Total anthocyanin contents in the flower petals of GST1 genome-edited lines #3 and #12 and the WT control. Quantities were estimated based on the molar absorptivity of delphinidin [ε mol = 506,783 at 530 nm, evaluated in an 80% methanol solution containing 0.1% (v/v) trifluoroacetic acid]. Error bars represent the standard error of the means of four petals. Different letters indicate statistically significant differences according to Tukey’s HSD test (P < 0.01)