Table 3. Differentially expressed genes in biofilm formation related KEGG pathways in L. plantarum RS66CD biofilm compared to planktonic cells.
| Term | Gene ID | Name | Log2FC | Type | Function |
|---|---|---|---|---|---|
| D-Alanine metabolism | |||||
| gene1728 | dltA | 1.32 | up | D-alanine ligase | |
| gene2020 | ddl | 1.39 | up | D-ala ligase | |
| gene433 | alr | 1.69 | up | Alanine racemase | |
| gene1726 | dltC | 1.37 | up | D-alanine ligase | |
| Peptidoglycan biosynthesis | |||||
| gene422 | murA | 1.44 | up | Carboxyvinyl transferase | |
| gene1094 | dacA | 2.49 | up | D-alanyl-D-alanine Carboxypeptidase | |
| gene1877 | mraY | 1.65 | up | Pentapeptide transferase | |
| gene2020 | ddl | 1.39 | up | D-ala ligase N-terminal domain protein | |
| gene707 | murB | 1.20 | up | UDP-pyruvate reductase | |
| gene1075 | lp-1256 | 1.59 | up | Hypothetical protein | |
| Two-component system | |||||
| gene508 | lytR | 1.76 | up | LytR family transcriptional regulator | |
| gene1358 | glnA | −1.70 | down | Type I glutamate-ammonia ligase | |
| gene939 | citG | 1.39 | up | Triphosphoribosyl-dephospho-CoA synthase | |
| gene455 | ftsH | 1.43 | up | ATP-dependent metallopeptidase | |
| gene3028 | lamA | 1.51 | up | DNA-binding response regulator | |
| gene2569 | lp-3019 | 2.15 | up | Membrane protein | |
| gene2757 | lrgB | 1.63 | up | Murein hydrolase | |
| gene2756 | lrgA | 2.65 | up | Murein hydrolase | |
| gene3031 | lamB | 1.89 | up | Accessory gene regulator AgrB | |
| gene2603 | hpk | 2.02 | up | Histidine protein kinase | |
| gene635 | pstF | −1.28 | down | Phosphate ABC transporter substrate-binding protein | |
| gene963 | cydA | −1.93 | down | Cytochrome D ubiquinol oxidase | |
| gene957 | mleS | 4.65 | up | NAD-dependent malic enzyme | |
| gene2658 | lp-3125 | 3.52 | up | Oxidoreductase | |
| gene964 | cydB | −1.79 | down | Cytochrome d ubiquinol oxidase | |
| gene682 | rpoN | −1.90 | down | RNA polymerase factor | |
| gene4 | gyrB | 0.83 | up | Ica regulatory factor | |
| Carbon metabolism | |||||
| gene2989 | tkt4 | −1.99 | down | Transketolase | |
| gene418 | sdhB | 1.44 | up | L-serine dehydratase | |
| gene419 | sdhA | 1.31 | up | L-serine dehydratase | |
| gene163 | serC | 1.93 | up | Phosphoserine aminotransferase | |
| gene162 | serA | 1.80 | up | D-3-phosphoglycerate dehydrogenase | |
| gene1635 | pps | −1.58 | down | Phosphoenolpyruvate synthase | |
| gene1840 | pdhC | −1.59 | down | Branched-chain α-keto acid dehydrogenase | |
| gene1841 | pdhB | −1.50 | down | α-keto acid dehydrogenase | |
| gene1842 | pdhA | −1.19 | down | Pyruvate dehydrogenase | |
| gene1839 | pdhD | −1.51 | down | Dihydrolipoyl dehydrogenase | |
| gene1534 | pgm5 | −1.17 | down | Histidine phosphatase family protein | |
| Bacterial secretion system | |||||
| gene3155 | traK | 2.47 | up | Conjugal transfer protein | |
| gene517 | radA | 1.64 | up | DNA repair protein RadA | |
| gene906 | secY | 1.35 | up | Preprotein translocase subunit |
Notes.
a
Log2FC: fold change in L. plantarum RS66CD biofilm compared to planktonic cells.