Figure 1.
Identification of LS-3 from Lumazine Synthase as a Potent CD4-Helper Epitope in C57BL/6 Mice
Mice received 25 μg DNA vaccination with EP twice 3 weeks apart and were euthanized 2 weeks post the second vaccination for cellular analysis.
(A) CD4+ T cell IFNγ responses induced to the 3BVE, LS, and PfV domains by DLnano_3BVE_GT8, DLnano_LS_GT8, and DLnano_PfV_GT8 vaccinations in BALB/c mice.
(B) Comparison of CD4+ cytokine responses to the LS domain induced by DLnano_LS_GT8 in BALB/c versus C57BL/6 mice.
(C) Comparison of polyfunctional CD4+ T cell responses to the LS domain induced by DLnano_LS_GT8 in BALB/c versus C57BL/6 mice.
(D and E) Matrix mapping by IFNγ ELISpot assays (D) and ICS (E) to determine HLA I-Ad CD4+ T cell epitopes in the LS domain in BALB/c mice immunized with DLnano_LS_GT8.
(F and G) Matrix mapping by IFNγ ELISpot assays (F) and ICS (G) to determine HLA I-Ab CD4+ T cell epitopes in the LS domain in C57BL/6 mice immunized with DLnano_LS_GT8. Each group includes five mice; each dot represents an animal; error bar represents standard deviation; two-tailed Mann-Whitney rank test used to compare groups; p values were adjusted for multiple comparison where appropriate; ∗, p value<0.05.