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. 2020 Jul 20;130(8):4486–4500. doi: 10.1172/JCI136564

Figure 1. MKRN3 mRNA levels in the mediobasal hypothalamus (MBH) decrease before puberty initiation in different species.

Figure 1

Expression of Mkrn3 mRNA in the hypothalamus of the female rat (A) or rhesus monkey (B) as determined by RT-qPCR. RNA expression data were normalized using peptidylprolyl isomerase A (Ppia) as the housekeeping gene and by dividing each individual value by the average of the P7 group for rats or the <6m group for monkeys. Bars represent mean ± SEM. In A, groups are as follows: 7 days old (P7; n = 8), 14 days old (P14; n = 7), 21 days old (P21; n = 4), 28 days old (P28; n = 5), and 32–34 days old (P32–34; n = 5). In B, groups are as follows: monkeys younger than 6 months of age (<6m; n = 4), 6 months to 1 year of age (6m–1y; n = 4), 1 to 2.5 years of age (1–2.5y; n = 7), 2.5 to 4 years of age (2.5–4y; n = 8). Mkrn3 mRNA levels decreased from P14 to P21 in the hypothalamus of rats (A), and from age 6m–1y to age 1.5–2y in the hypothalamus of monkeys (B). Groups with different symbols (†, ‡) are significantly different (A, P = 0.001; B, P = 0.046), as determined by 1-way ANOVA followed by the Student-Newman-Keuls test.