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. 2020 Jul 20;130(8):4486–4500. doi: 10.1172/JCI136564

Figure 5. MKRN3 inhibits KISS1 and TAC3 promoter activity.

Figure 5

Effect of MKRN3 on luciferase activity regulated by the human KISS1 (A), TAC3 (B), and PDYN (C) promoter (p). Effect of short truncated MKRN3 (sMKRN3) on KISS1 (D) and TAC3 (E) promoter activity. Neuro-2a cells were transfected with luciferase reporter constructs containing the 5′ flanking region of the indicated genes, in addition to an expression vector encoding WT MKRN3-HA or MKRN3 mutants. Forty-eight hours later, the cells were harvested and assayed for luciferase activity. Bars represent mean ± SEM (n = 4–6). (A–C) Although WT MRKN3 inhibited KISS1 and TAC3 (P = 0.0056) promoter activity, it did not inhibit PDYN promoter activity. (D and E) A truncated MKRN3 (sMKRN3) with 102 amino acids did not inhibit KISS1 or TAC3 promoter activity. Yellow bars represent the indicated gene promoters only, blue bars represent the indicated gene promoters and cotransfection with MKRN3, pink when both MKRN3 and sMKRN3 were cotransfected, and green when only sMKRN3 was cotransfected. Groups with different symbols (†, ‡, §, #, ¶) are significantly different (P < 0.05), as determined by 1-way ANOVA followed by the Student-Newman-Keuls test.