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. 2020 Jul 20;130(8):4440–4455. doi: 10.1172/JCI131721

Figure 3. Treg deficiency is not sufficient to compromise sarcolemmal resealing.

Figure 3

FDB muscles isolated from indicated mice and injured with an infrared laser in the presence of FM4-64 dye. The kinetics of sarcolemma resealing was measured by acquisition of images every 3 seconds for 60 seconds and calculation of the change in fluorescence before and after injury. (A) Representative images of C57BL, Foxp3–/Y, and Syt7–/– FDB muscles before (0 seconds) and after (60 seconds) injury. Scale bars: 20 μm. Yellow arrowheads indicate sites of injury. (B) Curves depicting mean sarcolemmal resealing kinetics measured every 3 seconds for 60 seconds. Data are represented as mean ± SEM. (C) AUC calculations representing total dye influx over time (n = 6, 15, and 5 for C57BL, Foxp3–/Y, and Syt7–/–, respectively; ANOVA, F(2,23) = 48.52, P < 0.0001; Tukey’s HSD: C57BL vs. Foxp3–/Y, P = 0.9413; C57BL vs. Syt7–/–, P < 0.0001; Syt7–/– vs. Foxp3–/Y, P < 0.0001). Data are represented as mean ± SD.