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. 2020 Jun 22;130(8):4081–4093. doi: 10.1172/JCI136727

Figure 4. Deletion of MFSD7c does not result in ruptured CNS blood vessels and increased permeability of BBB.

Figure 4

(A and B) Brain sections of E14.5 (n = 3 embryos per genotype) (A) and E16.5 (n = 4 embryos per genotype) (B). WT and Mfsd7c-KO embryos were stained with MFSD2a (a CNS endothelial cell marker, green) and Ter119 (red blood cell marker, pink). Arrows indicate clumps of erythrocytes surrounded by blood vessels in Mfsd7c-KO embryos. There was no hemorrhage in KO embryonic brains. Shown here are hindbrain regions. Scale bars: 20 μm. Experiments were repeated 3 times. (C and D) Representative coronal brain sections of E15.5 WT and KO showing that NHS-biotin was contained inside KO blood vessels. Arrows show enlarged blood vessels with strong signals of NHS-biotin. Experiments were repeated twice with n = 6 for WT or KO.