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. 2020 Jul 20;130(8):4501–4515. doi: 10.1172/JCI136745

Figure 2. Isl1Prop1KO cysts expand with age and have characteristics of hRCCs.

Figure 2

(A–I) Pituitary gland sections from control and Isl1Prop1KO mutants collected at 7 weeks (control, n = 4; Isl1Prop1KO, n = 10), 18 weeks (control, n = 3; Isl1Prop1KO, n = 4), and 1 year (control, n = 2; Isl1Prop1KO, n = 2) were stained with H&E. Multiple cysts are evident throughout adulthood in Isl1Prop1KO mutants. Cysts contain ciliated epithelium (C), nonciliated epithelium (NC), or a combination of ciliated and nonciliated epithelium (C/NC). (J–L) Immunostaining for cytokeratin 8 (KRT8), acetylated tubulin (TUBA1A), and FOXJ1 at 7 weeks detected expression of characteristic markers of hRCCs in Isl1Prop1KO mutants (n = 3). (M–O) Alcian blue/PAS staining detects mucosal cell (arrows) lining within the cyst walls from weaning (3 weeks, n = 1) through adulthood (7 weeks, n = 7; 18 weeks, n = 3) in Isl1Prop1KO mutants. Scale bars: 50 μm (A, D, M–O); 100 μm (B, C, E–L).