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. 2020 Jun 22;130(8):3974–3986. doi: 10.1172/JCI126866

Figure 4. Expression of the 3 key regulators in macrophages.

Figure 4

(A) Colocalization of CD68 with the 3 key regulators. Double immunofluorescence staining was performed in mouse synovial tissues at the resolution phase using Abs against CD68, and Itgb1 (left), Rps3 (middle), or Ywhaz (right). Scale bars: 10 μm. Data are representative of at least 3 independent experiments with similar results. (B and C) Determination of mRNA (B) and protein (C) expression levels of the 3 regulators in undifferentiated (M0) and differentiated M1 and M2 macrophages using qRT-PCR and Western blotting, respectively. Target gene and protein expression levels were normalized by those of Gapdh and β-actin, respectively. Data are the mean ± SEM (n = 4–7). *P < 0.05, **P < 0.01, ***P < 0.001 as determined by 1-way ANOVA with a post hoc test (Tukey’s correction).