Figure 2. CTNNBL1 M446V mutation decreases AID association with CTNNBL1.

(A) Lysates of EBV BLCLs from a healthy donor (HD), CTNNBL1 466^V/V patient, or AID-deficient patient were immunoprecipitated with an anti-CTNNBL1 antibody; precipitates and total cell lysates were analyzed by Western blotting with the indicated antibodies (shown are representative blots). (B) Summary of densitometric quantification of at least 6 independent experiments. Represented are values relative to HDs, indicated by dashed lines; bars represent the mean. (C) Lysates of parental WT, CTNNBL1 466^V/V, and AID^–/– Ramos B cells were analyzed as in A (shown are representative blots). (D) Summary of densitometric quantification of 5 independent experiments. Represented are values relative to parental WT cells, indicated by dashed lines; bars represent the mean.