Figure 3. CTNNBL1 M446V mutation interferes with CTNNBL1 thermal stability and reduces AID nuclear localization.

(A) Thermal treatment of parental WT, CTNNBL1 466^V/V, and AID^–/– Ramos B cells at indicated temperatures; nuclear lysates were analyzed by Western blotting with anti-CTNNBL1 antibody. (B) Nuclear and cytosolic lysates of WT, CTNNBL1 466^V/V, and AID^–/– Ramos B cells were analyzed by Western blotting with the indicated antibodies and (C) 4 independent experiments were quantified by densitometry. Represented are values relative to those of parental WT control indicated by dashed lines. Shown are representative blots from at least 3 independent experiments. Western blot of AID shows different exposures for nuclear and cytosolic fractions; bars represent the mean.