Figure 2. Dietary salt induces vasoconstriction with Rho activation under Klotho deficiency.

(A) Vasoconstrictive responses to U46619, a thromboxane A₂ analog, in the iliac arteries of young WT, aged WT, and heterozygous KL-KO mice receiving NS diet, HS diet, or HS diet + KL. n = 6 each. *P < 0.05 vs. HS diet–fed mice. (B) Vasoconstrictive response to phenylephrine was not affected by HS in the iliac arteries of young WT, aged WT, and young KL-KO mice fed NS diet or HS diet. n = 6 each. No significant differences were observed between HS diet– and NS diet–fed mice in all groups. (C) p-MYPT1 expression in response to U46619 in the iliac arteries of aged WT mice and KL-KO mice fed NS or HS diets, relative to the level in young WT mice. n = 9 each. *P < 0.05 vs. young WT, aged WT, or KL-KO fed NS diet. (D) p-MYPT1 expression in response to U46619 in the iliac arteries of aged WT and KL-KO mice receiving NS diet, HS diet, or HS diet + KL. n = 6 each. *P < 0.05 vs. HS. Data are summarized as means ± SEM. Unpaired t tests were used for comparisons between 2 groups. For multiple comparisons, statistical analysis was performed using the Tukey-Kramer post hoc test. N.S., not significant.