Figure 7. BACE1 activity and Aβ42 regulate aortic NO signaling.

(A) Representative immunoblots of p-eNOS(Ser¹¹⁷⁷), eNOS, p-PKB(Ser⁴⁷³), PKB, p-AMPK(Thr¹⁷²), AMPK, PKG, p-VASP(Ser²³⁹), ICAM1, and actin in aortas of ScrP- and Aβ42-treated RC-fed (left panel) and HF-fed (5 weeks) WT mice (middle panel) and in aortas of vehicle- or M-3–treated DIO mice (right panel). (B) Ratio of signal intensities for p-eNOS to eNOS, p-PKB to PKB, p-AMPK to AMPK; and for PKG, p-VASP, and ICAM1 to actin (n = 5–14). Data are means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 by Mann-Whitney U test.