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. 2020 Jun 22;130(8):4025–4038. doi: 10.1172/JCI134816

Figure 5. NMO-IgG induces microglial phenotypic changes.

Figure 5

(A) CD68 staining in Cx3cr1gfp/+ mice at L4 spinal cord level on day 5 of control-IgG (top) or NMO-IgG (bottom) infusion. n = 5 mice (4 sections/mouse). Scale bar: 200 μm. (B) Bar graph quantifies Cd68+ microglia cell number in both IgG recipient groups. n = 5 mice (4 sections/mouse). (C) Dual staining reveals complement C1q immunoreactivity (red) and Iba1 immunoreactivity (green) in L4 spinal cord cells on day 5 of IgG infusion. The arrowheads indicate colocalization of C1q with Iba1 staining. n = 5 mice (4 sections/mouse). Scale bar: 20 μm. (D) Bar graph quantifies C1q+ microglia percentage in both IgG recipient groups. n = 5 mice (4 sections/mouse). Data presented as the mean ± SEM. ***P < 0.001 by 2-tailed Student’s t test (B and D).